The Healing Effects of Conditioned Medium Derived from Human Neonatal Fibroblast Cells on Diabetic Wound

Document Type : Original paper


1 Department of Obstetrics and Gynecology, Faculty of Health Sciences, Medical University of Lublin, Poland

2 Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

3 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran


Background and aim: Although many studies suggest that conditioned medium derived from stem cells can be effective in wound healing, the results are still challenging. Accelerating the wound healing in diabetic patients is one the most important topics currently being discussed. The aim of the research is to investigate the effects of conditioned medium derived from neonatal fibroblast cells on diabetic wound healing in male rats. Materials and methods: Neonatal fibroblast cells were isolated from foreskin and cultured in DMEM culture medium. Conditioned medium was harvested. Male rats were divide into control (without treatment) and experiment (treatment with conditioned medium) groups (n=5 in each group). Diabetes was induced in animals by intraperitoneal injection of streptozotocin (150 mg/kg), and a full-thickness skin defect model was generated. Wound healing was evaluated 7, 14 and 21 days after treatment using Image J software. Results: Significant decrease in wound area was observed only at 21 days after treatment in control group; However, wound area was significantly decreased 14 and 21 days after treatment compared to the day 0. Conclusion: Our findings show that the conditioned medium derived from neonatal fibroblast cells isolated for foreskin has accelerating effects on diabetic wound healing in animal model.


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Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
Pages 147-153
  • Receive Date: 10 September 2022
  • Revise Date: 20 September 2022
  • Accept Date: 03 October 2022