In vitro Cytotoxic Effects of Sertraline on Normal Cells

Document Type : Original paper


1 Department of Clinical Psychology, Faculty of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

2 Department of Biology, Faculty of Advanced Sciences and Technologies, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

3 Department of Agricultural Sciences and Food Industry, Science and Research Branch, Islamic Azad University, Tehran, Iran


Background and aim: Studies have shown the cytotoxic effects of sertraline on various cells; however, the results are significantly contradictory. Accordingly, the present study investigated the cytotoxic effects of sertraline on normal human embryonic kidney (Hek293) cells. Materials and methods: In this experimental-laboratory study, Hek293 cells were divided into control group (no sertraline treatment) and sertraline treatment groups (1.56, 3.12, 6.25, 12.5, 25, 50 and 100 μg / ml). Cell viability was assessed by MTT 24 and 48 hours after sertraline treatment. Data were analyzed using one-way analysis of variance. Results: Treatment with 1.56 and 3.12 μg / ml of sertraline for 24 hours had no significant effect on the Hek-293 cells viability, however, the concentrations ≥6.25 μg / ml of sertraline significantly reduced the cell viability. Treatment of cells for 48 hours significantly reduced cell viability at concentrations ≥3.12 μg / ml of sertraline. Conclusion: The results of this study showed that sertraline can have a cytotoxic effect on non[1]cancerous cells and this effect depends on the dose and duration of treatment. This finding is important from sertraline side effects points of view on normal cells of the body considering its dose and period of consumption


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Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
  • Receive Date: 03 October 2022
  • Revise Date: 10 October 2022
  • Accept Date: 15 October 2022