Exploring the Impact of 5FU and Environmentally-Synthesized Silver Chloride Nanoparticles on Gastric Cancer Cells

Background and aim: Extensive research has highlighted the potential cytotoxic effects of nanoparticles and chemotherapy agents on cancer cells. However, the synergistic effects arising from the simultaneous utilization of silver chloride nanoparticles and 5FU in the treatment of gastric cancer cells remain insufficiently understood. In consideration of this knowledge gap, the current study endeavors to elucidate the cytotoxic outcomes of silver chloride nanoparticles and 5FU on gastric cancer cells, while also delving into the combined impact of these therapeutic interventions.
Materials and methods: In this experimental study, gastric cancer cells were categorized into distinct groups: control (untreated), treated with silver chloride nanoparticles alone, treated with 5FU alone, and subjected to the combined treatment of silver chloride nanoparticles and 5FU. Cell viability was assessed using the MTT method at 24 and 48-hour intervals post-treatment. The gathered data were subjected to analysis via one-way analysis of variance.
Results: The study reveals enhanced cytotoxic effects with the combined treatment of silver chloride nanoparticles and 5FU on AGS cancer cells compared to 5FU alone. Lower concentrations of 5FU exhibit limited impact on cell viability, whereas the addition of silver chloride nanoparticles amplifies the reduction in viability, indicating a potential synergy.
Conclusion: The findings of this investigation emphasize that the concurrent application of silver chloride nanoparticles exerts heightened cytotoxic effects on gastric cancer cells in comparison to the use of 5FU alone.