Document Type : Original paper

Author

• Nima RabieNezhadganji

Department of Biopathology and Medical Biotechnology, Faculty of Oncology and Surgery , University of Palermo, Palermo, Italy

Abstract

Studies have shown that sex steroid hormones affect the proliferation of cancer cells at the cellular and molecular levels. This study aimed to evaluate the effect of estradiol valerate and progesterone on caspase 8 and 9 activity and expression of BAX, BCL2, and P53 genes in HT29 cells in a cell culture medium. Cell line was treated with estradiol (1 mg/ml) and progesterone (0.01 mg/ml). Also, the expression of BAX, BCL2, and P53 genes was measured using real-time PCR and at the end of caspases -8 and -9 activity by specialized ELISA kits. Data were also compared between groups using an independent t-test. BAX gene expression increased significantly compared to the control group (P <0.001); on the other hand, BCL gene expression did not differ significantly (P <0.05). Based on this, it can be concluded that the path of action of estradiol valerate on HT29 cells is through internal apoptosis, and progesterone is through extracellular apoptosis. Data were also compared between groups using an independent t-test. BAX gene expression increased significantly compared to the control group (P <0.001); on the other hand, BCL gene expression did not differ significantly (P <0.05). Based on this, it can be concluded that the path of action of estradiol valerate on HT29 cells is through internal apoptosis, and progesterone is through extracellular apoptosis.

Keywords

• BAX
• BCL2
• P53
• HT29
• Caspase-8
• 9

Main Subjects

• Cancer biology