Isolation and Characterization of Human Adipose Tissue Derived Mesenchymal Stem Cells

Document Type : Original paper

Authors

1 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Abstract

Background and aim: Mesenchymal stem cells are a group of stem cells that have attracted a great deal of attention from researchers for the treatment of malignant diseases due to their inherent ability to differentiate into different cell lines. There have been numerous studies on the biological properties of Mesenchymal stem cells, but studies on the biological properties of Adipose-derived Mesenchymal stem cells are limited, particularly at the cellular and molecular level. In this study, we investigate the biological properties of Adipose tissue derived Mesenchymal stem cells at the cellular and molecular levels. Materials and methods: In this experimental in vitro study, Adipose tissue was obtained from 10 patients and transferred to a cell culture laboratory and kept under standard conditions. Adipose cells were cultured in high glucose DMEM supplemented with 10% FBS culture medium. Finally, with the preparation of cells, their biological properties, such as surface cell markers, were analyzed by flow cytometry and finally the data were analyzed by statistical methods. Results: In these cells, CD105-PE, CD73-PE, CD90-PE expression was over 95% and CD105-PE expression was less than 2% which follows the pattern consistent with the expression of CDs in MSCs. Adipose-derived cells had also the potential of adipogenic and osteogenic differentiation. Conclusion: Morphology, cell cycle, expression of cell surface markers and differential potential in adipose-derived cells were similar to MSCs.

Keywords

References

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International Journal of BioLife Sciences (IJBLS)
Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
Pages 193-200

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History

  • Receive Date: 28 September 2022
  • Revise Date: 02 October 2022
  • Accept Date: 09 October 2022

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