AgCl Nanoparticles in vitro Anticancer Effects Against Human Cervical Cancer Cells

Document Type : Original paper


1 Aldent University, Tirana, Albania

2 Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran


Background and aim: Nanoparticles have potential anticancer effects on cancer cells. In vitro and in vivo studies have reported the cytotoxic effects of silver nanoparticles on cancer cells. Despite a number of studies carried out on the anticancer effects of silver nanoparticles on cancer cells, there are few report on the anticancer effects of green synthesized silver nanoparticles on cervical cancer cells. The aim of this study was to determine the cytotoxic effects of green synthesized AgCl on cervical cancer (Hela) cells in vitro. Materials and methods: AgCl nanoparticles were synthesized using Onopordum acanthium extract. HeLa cells were divided into control (no- treated) group and groups treated with 1.5625, 3.125, 6.25, and 12.5µg/ml of AgCl nanoparticles. Cell viability was evaluated using MTT assay method. Results: The results of MTT showed that viability of Hela cells did not significantly change in groups treated with 1.5625 and 3.125 µg/ml of AgCl nanoparticles. However, the Hela cells viability significantly decreased compared to the control group at concentrations ≥ 6.25 µg/ml of AgCl nanoparticles. Conclusion: Our findings revealed that despite lower concentrations of AgCl nanoparticles, higher concentrations had cytotoxic effects on cervical cancer cells in vitro.


[1]. Ravindran A, Chandran P, Khan SS. Biofunctionalized silver nanoparticles: advances and prospects. Colloids and Surfaces B: Biointerfaces. 2013;105:342-52.
[2]. Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein journal of nanotechnology. 2018;9(1):1050-74.
[3]. Rajeshkumar S. Anticancer activity of eco-friendly gold nanoparticles against lung and liver cancer cells. Journal of Genetic Engineering and Biotechnology. 2016;14(1):195-202.
[4]. Daei S, Ziamajidi N, Abbasalipourkabir R, Khanaki K, Bahreini F. Anticancer effects of gold nanoparticles by inducing apoptosis in bladder cancer 5637 cells. Biological Trace Element Research. 2022;200(6):2673-83.
[5]. Joshi P, Chakraborti S, Ramirez-Vick JE, Ansari ZA, Shanker V, Chakrabarti P, Singh SP. The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells. Colloids and Surfaces B: Biointerfaces. 2012;95:195-200.
[6]. Daduang J, Palasap A, Daduang S, Boonsiri P, Suwannalert P, Limpaiboon T. Gallic acid enhancement of gold nanoparticle anticancer activity in cervical cancer cells. Asian Pacific Journal of Cancer Prevention. 2015;16(1):169-74.
[7]. Castro-Aceituno V, Ahn S, Simu SY, Singh P, Mathiyalagan R, Lee HA, Yang DC. Anticancer activity of silver nanoparticles from Panax ginseng fresh leaves in human cancer cells. Biomedicine & Pharmacotherapy. 2016;84:158-65.
[8]. Arbyn M, Weiderpass E, Bruni L, de Sanjosé S, Saraiya M, Ferlay J, Bray F. Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. The Lancet Global Health. 2020;8(2):e191-203.
[9]. Wang L, Guo H, Lin C, Yang L, Wang X. Enrichment and characterization of cancer stem-like cells from a cervical cancer cell line. Molecular medicine reports. 2014;9(6):2117-23.
[10]. Santos PA, Avanço GB, Nerilo SB, Marcelino RI, Janeiro V, Valadares MC, Machinski M. Assessment of cytotoxic activity of rosemary (Rosmarinus officinalis L.), turmeric (Curcuma A. Tabaku et al. / Intl J of BiolIfe Sciences: 1(3) 182-186, 2022186 longa L.), and ginger (Zingiber officinale R.) essential oils in cervical cancer cells (HeLa). The Scientific World Journal. 2016;2016.
[11]. Mfouo-Tynga I, El-Hussein A, Abdel-Harith M, Abrahamse H. Photodynamic ability of silver nanoparticles in inducing cytotoxic effects in breast and lung cancer cell lines. Int J Nanomed. 2014;9(1):3771-80.
[12]. Selvi BC, Madhavan J, Santhanam A. Cytotoxic effect of silver nanoparticles synthesized from Padina tetrastromatica on breast cancer cell line. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2016;7(3):035015.
[13]. Chengzheng W, Jiazhi W, Shuangjiang C, Swamy MK, Sinniah UR, Akhtar M, Umar A. Biogenic synthesis, characterization and evaluation of silver nanoparticles from Aspergillus niger JX556221 against human colon cancer cell line HT-29. Journal of nanoscience and nanotechnology. 2018;18(5):3673-81.
[14]. Yuan YG, Peng QL, Gurunathan S. Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment. International journal of nanomedicine. 2017;12:6487.
[15]. He Y, Du Z, Ma S, Cheng S, Jiang S, Liu Y, Li D, Huang H, Zhang K, Zheng X. Biosynthesis, antibacterial activity and anticancer effects against prostate cancer (PC-3) cells of silver nanoparticles using Dimocarpus Longan Lour. peel extract. Nanoscale Research Letters. 2016;11(1):1-0.
[16]. Prabakaran K, Ragavendran C, Natarajan D. Mycosynthesis of silver nanoparticles from Beauveria bassiana and its larvicidal, antibacterial, and cytotoxic effect on human cervical cancer (HeLa) cells. RSC advances. 2016;6(51):44972-86.
[17]. Suganya S, Dhanalakshmi B, Dinesh Kumar S, Santhanam P. Cytotoxic effect of silver nanoparticles synthesized from Sargassum wightii on cervical cancer cell line. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2020;90(4):811-8.
[18]. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Al-Massarani SM, Saquib Q, Wahab R, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Anticancer potential of green synthesized silver nanoparticles using extract of Nepeta deflersiana against human cervical cancer cells (HeLA). Bioinorganic Chemistry and Applications. 2018;2018.
[19]. Adebayo IA, Arsad H, Gagman HA, Ismail NZ, Samian MR. Inhibitory effect of eco-friendly naturally synthesized silver nanoparticles from the leaf extract of medicinal Detarium microcarpum plant on pancreatic and cervical cancer cells. Asian Pacific journal of cancer prevention: APJCP. 2020;21(5):1247.
[20]. Akter M, Atique Ullah AK, Banik S, Sikder M, Hosokawa T, Saito T, Kurasaki M. Green synthesized silver nanoparticles-mediated cytotoxic effect in colorectal cancer cells: NF-κB signal induced apoptosis through autophagy. Biological Trace Element Research. 2021;199(9):3272-86.
[21]. Zhang XF, Gurunathan S. Combination of salinomycin and silver nanoparticles enhances apoptosis and autophagy in human ovarian cancer cells: an effective anticancer therapy. International journal of nanomedicine. 2016;11:3655
Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
Pages 182-186
  • Receive Date: 18 September 2022
  • Revise Date: 04 October 2022
  • Accept Date: 12 October 2022