Document Type : Original paper
1 Aldent University, Tirana, Albania
2 Department of Biology, Faculty of Basic Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran
Background and aim: Nanoparticles have potential anticancer effects on cancer cells. In vitro and in vivo studies have reported the cytotoxic effects of silver nanoparticles on cancer cells. Despite a number of studies carried out on the anticancer effects of silver nanoparticles on cancer cells, there are few report on the anticancer effects of green synthesized silver nanoparticles on cervical cancer cells. The aim of this study was to determine the cytotoxic effects of green synthesized AgCl on cervical cancer (Hela) cells in vitro. Materials and methods: AgCl nanoparticles were synthesized using Onopordum acanthium extract. HeLa cells were divided into control (no- treated) group and groups treated with 1.5625, 3.125, 6.25, and 12.5µg/ml of AgCl nanoparticles. Cell viability was evaluated using MTT assay method. Results: The results of MTT showed that viability of Hela cells did not significantly change in groups treated with 1.5625 and 3.125 µg/ml of AgCl nanoparticles. However, the Hela cells viability significantly decreased compared to the control group at concentrations ≥ 6.25 µg/ml of AgCl nanoparticles. Conclusion: Our findings revealed that despite lower concentrations of AgCl nanoparticles, higher concentrations had cytotoxic effects on cervical cancer cells in vitro.
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