The Healing Effects of Human Acellular Amniotic Membrane on Skin Wound in Diabetic Animals

Document Type : Original paper


1 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Biology, Faculty of Basic Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran


Background and aim: Human amniotic membrane has been used as a versatile skin alternative in dermatology as an ideal biological dressing material. It has been reported to have wound healing effects, however, the findings still are challenging. The aim of this study was to investigate the healing effects of acellular human amniotic membrane on skin wound healing in diabetic rats. Materials and methods: Acellular amnion membrane was prepared using DMSO and characterized by electron microscopy. Rats were divide into control (without treatment) and experiment (treatment with acellular amniotic membrane) groups (n=5 in each group). Diabetes was induced in animals by intraperitoneal injection of streptozotocin (150 mg/kg), and a full[1]thickness skin defect model was generated. Wound healing was evaluated 7, 14 and days after treatment using Image J software. Results: Significant decrease in wound area was observed 7, 14 and 21 days after treatment in experiment rats compared with control animals. The was also significant difference between wound area at 7, 14 and 21 days after treatment. Conclusion: Our findings indicated that acellular human amniotic membrane has a significant wound healing potential, accelerating the wound healing process in diabetic subjects.


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Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
Pages 159-164
  • Receive Date: 11 September 2022
  • Revise Date: 15 September 2022
  • Accept Date: 05 October 2022