The Cytotoxic Effects Biocompatible Nanobubbles Carrying Quercetin on Non-small-cell Lung Carcinoma Cells

Document Type : Original paper


1 Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

2 Polymer Laboratory, School of Chemistry, College of Science, University of Tehran, PO Box 14155 6455, Tehran, Iran


Background and aim: Non-small-cell lung carcinoma (NSCLC), as major lung cancer is currently considered as one of the leading causes of mortality and has become a progressively serious global public health burden. However, the conventional drug delivery approaches were unable to efficiently inhibit the proliferation and metastasis of the lung cancer cells. Exploiting nanobubbles (NBs) as a novel drug delivery system have recently a research hotspot mainly due to their outstanding characteristics such as small size, biosafety and competent drug-transporting ability. Present study aimed to establish a novel biocompatible approach for nanobubble constructions by the water-in-oil method and loading quercetin inside the obtained micelles. Materials and methods: Dextran coating was used for more stability of NBs and the effectiveness of drug delivery to A549 NSCLC cells was evaluated. Ultrasound waves were used to stimulate the nanobubble to release the drug. Excellent drug-loading capacity and ultrasound-mediated release of quercetin were confirmed by UV spectrophotometer with the absorption about 1.6. Results: NBs efficiently inhibited the proliferation of NSCLC cells in a concentration-dependent manner as well as the capability to achieve ultrasound enhancement. This experiment showed obtained NBs effectively delivered quercetin into lung cancer cells promoted by ultrasound irradiation. Conclusion: In conclusion, proposed biocompatible quercetin loaded NBs are suitable for ultrasound-targeted drug delivery and are thus a promising strategy for their noninvasive clinical application.


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Volume 1, Issue 3
(Special Issue: papers selected from ICLS22, Istanbul, Turkey)
Pages 141-146
  • Receive Date: 28 September 2022
  • Revise Date: 05 October 2022
  • Accept Date: 12 December 2022