International Journal of BioLife Sciences (IJBLS)

Document Type : Original paper


1 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

2 Department of Biology, Faculty of Advanced Sciences and Technologies, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

3 Department of Biology, Avicenna International College, Budapest, Hungary



Background and aim: 5-Fluorouracil (5FU) is one of the most commonly used drugs in chemotherapy for cancer treatment. In combination with other cancer drugs, it is used to treat many types of cancer including breast, colon, stomach and cervix cancer. However, treatment with 5FU is followed by serious side effects on healthy non-cancerous cells. The aim of this study was to investigate the cytotoxic effects of 5FU on cervical cancer (Hela) cells compared to non-cancerous (Hek293) cells. Materials and methods: In this experimental-laboratory study, HeLa and Hek293 cells were divided into control group (no- treated) and groups treated with 3.90625, 7.8125, 15.625, 31.25, 62.5, 125, 250 and 500 µg/ml of 5FU. MTT assay method was used to evaluate the cell viability. Results: The findings showed that 3.90625, 7.8125 and 15.625 µg/ml of 5FU did not significantly change cell viability, however, 31.25, 62.5, 125, 250 and 500 µg/ml of 5FU significantly decreased the Hek293 cell viability. Treatment of HeLa cells with 3.90625 and 7.8125 µg/ml of 5FU did not significantly change the cell viability, while HeLa cells treated with 15.625, 31.25, 62.5, 125, 250 and 500 µg/ml of 5FU showed a significant decrease in cell viability. Conclusion: In conclusion, high doses of 5FU has the same cytotoxic effects on HeLa and Hek293 cells, however, there was a narrow difference between 5FU cytotoxic effects on HeLa cancer cells compared to non-cancerous in lower doses indicating that 5FU concentration play a significant role in its cytotoxic action on healthy cells.


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